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paav gfap egfp (Addgene inc)

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Addgene inc paav gfap egfp
Paav Gfap Egfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/paav+gfap+egfp/pAAV-GFAP-EGFP+(Plasmid+%2350473)/pmc12765836-60-0-2
Average 93 stars, based on 40 article reviews

paav gfap egfp - by Bioz Stars, 2026-09

93/100 stars

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Plasmid Preparation:

Article Title: Activation of G<sub>s</sub>Signaling in Cortical Astrocytes Does Not Influence Formation of a Persistent Contextual Memory Engram
Article Snippet: .. The pAAV-GFAP::HA-rM3Ds-EGFP plasmid was generated from pAAV-GFAP::HA-rM3Ds-IRES-mCitrine (gift from Bryan Roth, Addgene plasmid #50472) by replacing IRES-mCitrine with EGFP from pAAV-GFAP-EGFP (gift from Bryan Roth lab, Addgene plasmid #50473). ..

Article Title: Activation of G s Signaling in Cortical Astrocytes Does Not Influence Formation of a Persistent Contextual Memory Engram
Article Snippet: .. The pAAV-GFAP::HA-rM3Ds-EGFP plasmid was generated from pAAV-GFAP::HA-rM3Ds-IRES-mCitrine (gift from Bryan Roth, Addgene plasmid #50472) by replacing IRES-mCitrine with EGFP from pAAV-GFAP-EGFP (gift from Bryan Roth lab, Addgene plasmid #50473). ..

Generated:

Expressing:

Article Title: Hippocampal astrocytes induce sex-dimorphic effects on memory
Article Snippet: .. To enhance mGluR3 expression levels, the truncated human astrocyte-specific GfaABC1D promoter was digested from pAAV- GFAP -EGFP (donated by Dr. Bryan Roth, Addgene #50473; RRID #Addgene_50473) and cloned into pAAV- EF1a -DIO-hM4D(Gi)-mCherry (donated by Dr. Bryan Roth, Addgene #50461; RRID #Addgene_50461) using Anza 28 Mlul and Anza 14 SalI to replace the EF1a promoter. .. Rat mGluR3 with an N-terminal HA tag was synthesized as a gene block (Integrated DNA Technologies) and ligated in place of the hM4D(Gi) sequence of pAAV- GfaABC1D -DIO-hM4D(Gi)-mCherry using Anza 6 NheI and Anza 21 SgsI.

Clone Assay:

Sequencing:

Article Title: RAB12-LRRK2 Complex Suppresses Primary Ciliogenesis and Regulates Centrosome Homeostasis in Astrocytes
Article Snippet: .. An HA tag was added to the N terminal of the m Rab 12WT coding sequence and subcloned into pAAV- GFAP-EGfp (addgene, #50473) using SalI and EcoRI restriction enzyme sites and LV- hGFAP-Gfp (addgene, #183906) using AgeI and XhoI restriction enzyme sites, respectively. ..

Article Title: RAB12-LRRK2 complex suppresses primary ciliogenesis and regulates centrosome homeostasis in astrocytes
Article Snippet: .. An HA tag was added to the N terminal of the m Rab 12 coding sequence and subcloned into pAAV- GFAP-EGfp (addgene, #50473) using SalI and EcoRI restriction enzyme sites and LV- hGFAP-Gfp (addgene, #183906) using AgeI and XhoI restriction enzyme sites, respectively. ..